Evaluation of toxicity, local biocompatibility, biodegradation, and systemic metabolism of cellulose/alginate/strontium apatite membranes implanted subcutaneously in mice

Evaluation of toxicity, local biocompatibility, biodegradation, and systemic metabolism of cellulose/alginate/strontium apatite membranes implanted subcutaneously in mice

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ABSTRACT Purpose: To evaluate membranes originating from pure or oxidized bacterial cellulose (BC)/alginate/strontium apatite hydrogels regarding toxicity, biocompatibility, biodegradation and metabolism.Methods: The toxicity was measured by incubating the materials with Artemia salina for 24 h, and mortality and the 50% lethal concentration were determined in comparison to potassium dichromate by Probit analysis.Local biocompatibility and biodegradation were evaluated by subcutaneous assay in 75 Swiss mice; the Goalie - Cages test groups were compared to sham and collagen membrane at one, three and nine weeks.The histopathology of tissue irritation followed the ISO 10993-6 standard, and the integrity of the biomaterials scored by quartiles.

Metabolic analysis of relative weight and the intensity of catalase, iodine and PWR REC Loveseat/CON/ADJ HDRST nitrite were carried out for liver, kidneys and tibias of the tested animals.Results: All cellulose-based materials were nontoxic, biocompatible, and none presented nitrosative stress.The oxidized BC was more resorbable, and the non-oxidized BC had greater renal biochemical reactivity.Conclusion: The membranes suggest applicability as regenerative barriers.

However, long-term studies in bone defects are necessary to elucidate their osteopromoting efficiency.